Live observation of Endothelial to Mesenchymal Transition.
I am a recent graduate engineer from Ecole des Mines de Saint-Etienne, France, where I had the opportunity to discover bioengineering. I spent two years in Canada completing a Master of Science (M.Eng) in Biomedical Engineering from Ecole Polytechnique, Montreal.
After this international experience, I got involved in a research project at Institut Pasteur, Paris, working on an Intestine-on-a-chip microfluidic model to investigate pathogens infection under mechanical forces.
I joined Eden Tech in Paris as ESR13 enrolled in the PhD program in Translational and Molecular Medicine (DIMET) within the Renoir consortium. The PhD project will aims to develop a microfluidic bioreactor capable of recapitulating the muscle niche in vitro. The device will provide a relevant platform to study, modulate and observe muscle cells environment/interactions such as endothelial to mesenchymal transition in live microscopy.
Eden Tech is a new start-up in Paris, member of the NBIC Valley consortium, on advanced polymers solutions and low-cost and ultra-compact microfabrication systems for microfluidics.
The NBIC Valley is a SME consortium specialized in microfluidics that supports each other through facility sharing, administrative support, and general mentoring. The NBIC valley includes a wide range of entities, including Black Hole Labs, Elvesys, Darwin Microfluidics and 4D cell.
The IP-protected technology of Eden Microfluidics stems from a decade of research on nanoblocks chemistry and polymer formulation for microfluidic applications.
Research-wise, EM develops materials for demanding applications, such as fluoro-elastomers for organ-on-chip and complex liquids (oils, corrosives solutions). It also develops simple, low-cost and easy to use microfabrication units that can be deployed in every lab to almost any public. Our vision is to spread microfluidics beyond its current niches, with compact, plug-and-play systems and versatile polymers. Within this context, we welcome researchers of all fields to train them in microfluidics in a dynamic entrepreneurship environment.
The ESR will be enrolled in the Ph.D. Programme in Translational and Molecular Medicine (DIMET), University of Milano Bicocca (www.dimet.org)
Several studies support the emerging concept that inflammation controls stem cell fate/behaviour coordinating tissue repair and this balance is probably skewed in patients with late phases of chronic diseases, like muscle dystrophies and in aging.
We will focus on how the inflammatory, myogenic and vascular components integrate to coordinate muscle regeneration and how Endothelial to Mesenchymal Transition (EndoMT) and fibrosis develops in pathological conditions. This will allow the identification of critical cell mediators and key factors produced by them that could be exploited in the cell and pharmacological therapy of muscle diseases.
We will design a dedicated live-imaging solution based on cutting-edge technologies to study EndoMT and cell-cell interactions in controlled conditions of cell growth, maintenance and medium injection/change over a long period of time.
Preferred profile of applicants: Background in mecatronics and/or microfluidic engineering.
To design a high-resolution imaging solution to image early stages mesenchymal transition using a combination of markers
To study through live-imaging mesenchymal transition after injection of specific population of leukocytes and specific biopharma
Ph.D. Programme in Translational and Molecular Medicine (DIMET),
University of Milano Bicocca