ESR8

Controlling cell microenvironment: engineering artificial niches to study muscle degeneration-regeneration in vitro.

University of Naples

University of Naples

Italy

Partner Profile
Paolo A. Netti
Paolo A. NettiSupervisor at University of Naples

Description

It is well known that extracellular stimuli from the microenvironment are crucial for cell adhesion, migration, proliferation and differentiation, and the ECM constitutes the very foundation of tissue homeostasis and development (Seliktar, 2012). Despite this, the analysis of such cells/ECM interplays in vivo is hampered by the intrinsic complexity of the native environment.

ESR8 will engineer bioactive materials and devices (Iannone et al., 2015) that can recapitulate in a simplified but consistent form the role of the biophysical/biochemical signals in muscle regeneration/degeneration. ESR8 will carry on a preliminary screen of the most relevant biochemical (adhesion motifs) or biophysical (mechanical or topographic) signaling patterns to define the optimal set of stimuli for the in vitro myoblast culture.

Artificially made scaffolds displaying adequate mechanical and biochemical features will be then fabricated by employing synthetic or hybrid materials. The micro- nano-scale architecture of these scaffolds will be controlled by state-of-the-art technologies (nano and microfabrication, or a combination of these). The effectiveness of signals along with their spatio-temporal presentation will be tested and validated.

ESR8 will reproduce in vitro the basal lamina niche of skeletal, muscle or blood vessel or a collagen sclerotic environment and will challenge different progenitors’ fate under these condition.

Objectives
  • To engineer bioactive materials and devices that can recapitulate the role of the extracellular matrix in muscle degeneration-regeneration (with ESR5)

  • To engineer bioactive materials for protein and factor delivery

Duration

36 months

Enrolment in Doctoral degree

PhD Programme in “Industrial product and process engineering” University of Naples “Federico II”.

References

Iannone, M., Ventre, M., Formisano, L., Casalino, L., Patriarca, E.J., and Netti, P.A. (2015). Nanoengineered surfaces for focal adhesion guidance trigger mesenchymal stem cell self-organization and tenogenesis. Nano Lett 15, 1517-1525.
Seliktar, D. (2012). Designing cell-compatible hydrogels for biomedical applications. Science (New York, NY) 336, 1124-1128.

APPLY HERE

RENOIR

0

ESR PROGRAMS

0

COUNTRIES

0

YEARS

0

MEMBERS

JOIN US

Meet Our Team

FIND OUT MORE

Latest News